Ruan Ackermann, Freddie Clarke, Chris Harris, Zach Mercer, Albert Tuisue and Mayco Vivas are among 19 players who will be leaving Gloucester at the end of this season.
Back row Ackermann and lock Clarke have both surpassed 150 appearances for the Cherry and Whites, with Scotland and British and Irish Lions centre Harris scoring 26 tries in 106 matches so far.
Fijian back row Tuisue has started six out of 17 matches this season and has been at Gloucester since 2022, along with Argentina loose-head prop Vivas.
Number eight Mercer, who signed in 2023 with the hope of returning to the England set-up, had his season ended by a knee injury in November and has been linked with a return to a French club.
Centre Louis Hillman-Cooper, prop Alfie Petch and hooker Morgan Nelson will also be leaving.
Versatile Argentina back Santiago Carreras had already confirmed his move to Bath for 2025-26, fly-half Gareth Anscombe has signed for French Top 14 side Bayonne, scrum-half Charlie Chapman will move to Premiership rivals Exeter and winger Christian Wade is switching codes to rugby league to join Wigan Warriors.
Academy players Morgan Adderly-Jones, Ioan Jones, Gareth Blackmore and Rory Taylor and dual-registered Matty Ward and Aristot Benz Salomon will also depart the club this summer.
