Uncapped Nikki Simpson and Holly McIntyre have been named in Scotland’s squad for their WXV training camp and warm-up match against Spain.
Sarah Bonar, Lisa Cockburn, Jenny Maxwell and Emma Wasssell all return from injury.
Great Britain Sevens players Shona Campbell, Rhona Lloyd and Lisa Thomson are also selected.
Scotland host Spain on 30 September and play their first WXV match against hosts South Africa on 13 October.
Bryan Easson’s side will also take on the United States on 20 October and Japan eight days later.
The new three-tiered global women’s tournament starts in October despite clashing with the men’s World Cup.
England, Wales and France are in the top-tier WXV1 after finishing in the top three of the Women’s Six Nations with Scotland in WXV2 alongside Italy and Samoa.
Scotland squad
Forwards: Leah Bartlett (Leicester Tigers), Christine Belisle (Loughborough Lightning), Sarah Bonar (Harlequins), Elliann Clarke (Bristol Bears), Lisa Cockburn (University of Worcester Warriors), Eva Donaldson (Leicester Tigers), Evie Gallagher (University of Worcester Warriors), Jade Konkel (Harlequins), Rachel Malcolm (Loughborough Lightning), Elis Martin (Leicester Tigers), Rachel McLachlan (Sale Sharks), Louise McMillan (Saracens), Lana Skeldon (University of Worcester Warriors), Nikki Simpson (Garioch), Emma Wassell (Loughborough Lightning), Anne Young (Sale Sharks)
Backs: Beth Blacklock (Saracens), Shona Campbell (GB Sevens), Coreen Grant (Saracens), Caity Mattinson (University of Worcester Warriors), Jenny Maxwell (Loughborough Lightning), Mairi McDonald (Exeter Chiefs), Francesca McGhie (Leicester Tigers), Holly McIntyre (University of Edinburgh), Rhona Lloyd (GB Sevens/Les Lionnes du Stade Bordelais), Liz Musgrove (Wasps), Helen Nelson (Loughborough Lightning), Emma Orr (Heriots/Biggar), Lisa Thomson (GB Sevens), Chloe Rollie (Loughborough Lightning), Eilidh Sinclair (Exeter Chiefs), Meryl Smith (Bristol Bears)